VHH antibodies: Emerging reagents for the analysis of ...
www.ncbi.nlm.nih.gov › pmc › articlesVHH had superior temperature stability and could bind antigen well at elevated temperature and higher concentrations of the chaotrope ammonium thiocyanate compared to mAbs. Both mAbs and VHHs showed no change in binding in the presence of up to 50% ethanol . Further examination of the crystal structure of a VHH revealed that although VHH lack the hydrophobic cleft typically formed by VL and VH chains, the CDRs of VHH are adaptable and binding may occur in any number of ways.
VHH antibodies: emerging reagents for the analysis of ...
pubmed.ncbi.nlm.nih.gov › 27209591A VHH antibody (or nanobody) is the antigen binding fragment of heavy chain only antibodies. Discovered nearly 25 years ago, they have been investigated for their use in clinical therapeutics and immunodiagnostics, and more recently for environmental monitoring applications. A new and valuable immunoreagent for the analysis of small molecular weight environmental chemicals, VHH will overcome many pitfalls encountered with conventional reagents.